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brdu cell proliferation kit 2750  (Millipore)

 
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    Structured Review

    Millipore brdu cell proliferation kit 2750
    Silencing of C/EBPβ and KIFC1 reduces <t>proliferation</t> in AR-TNBC cells. ( A – G ) Representative immunofluorescence images ( A – D ) and quantification bar graphs ( E – G ) showing <t>BrdU</t> (green) incorporation in AR + TNBC ( A , B ) and AR-TNBC ( C , D ) cells transfected with scrambled or C/EBPβ siRNA ( A – D , E ) or treated with CW069 ( A – D , G ). Nuclei were counterstained with Hoechst (blue) and α-tubulin (red). ( H – J ) Bar graphs showing BrdU incorporation in cells transfected with scrambled siRNA, C/EBP siRNA ( H ), KIFC1 siRNA ( I ), or treated with CW069 ( J ). Absorbance was measured at 450–540 nm. A : HCC70, B : MFM223, C : HCC1806, D : BT20; A , B -AR-positive TNBC, C , D-AR-negative TNBC. Bars indicate mean ± SEM. Unpaired two-tailed Student’s t -test with Welch’s correction was used to determine statistical significance. * P < 0.05, ** P < 0.005, ns = non-significant
    Brdu Cell Proliferation Kit 2750, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/brdu cell proliferation kit 2750/product/Millipore
    Average 90 stars, based on 1 article reviews
    brdu cell proliferation kit 2750 - by Bioz Stars, 2026-04
    90/100 stars

    Images

    1) Product Images from "C/EBPβ increases tumor aggressiveness by enhancing KIFC1 expression in androgen receptor negative triple negative breast cancer"

    Article Title: C/EBPβ increases tumor aggressiveness by enhancing KIFC1 expression in androgen receptor negative triple negative breast cancer

    Journal: Cell Communication and Signaling : CCS

    doi: 10.1186/s12964-025-02243-7

    Silencing of C/EBPβ and KIFC1 reduces proliferation in AR-TNBC cells. ( A – G ) Representative immunofluorescence images ( A – D ) and quantification bar graphs ( E – G ) showing BrdU (green) incorporation in AR + TNBC ( A , B ) and AR-TNBC ( C , D ) cells transfected with scrambled or C/EBPβ siRNA ( A – D , E ) or treated with CW069 ( A – D , G ). Nuclei were counterstained with Hoechst (blue) and α-tubulin (red). ( H – J ) Bar graphs showing BrdU incorporation in cells transfected with scrambled siRNA, C/EBP siRNA ( H ), KIFC1 siRNA ( I ), or treated with CW069 ( J ). Absorbance was measured at 450–540 nm. A : HCC70, B : MFM223, C : HCC1806, D : BT20; A , B -AR-positive TNBC, C , D-AR-negative TNBC. Bars indicate mean ± SEM. Unpaired two-tailed Student’s t -test with Welch’s correction was used to determine statistical significance. * P < 0.05, ** P < 0.005, ns = non-significant
    Figure Legend Snippet: Silencing of C/EBPβ and KIFC1 reduces proliferation in AR-TNBC cells. ( A – G ) Representative immunofluorescence images ( A – D ) and quantification bar graphs ( E – G ) showing BrdU (green) incorporation in AR + TNBC ( A , B ) and AR-TNBC ( C , D ) cells transfected with scrambled or C/EBPβ siRNA ( A – D , E ) or treated with CW069 ( A – D , G ). Nuclei were counterstained with Hoechst (blue) and α-tubulin (red). ( H – J ) Bar graphs showing BrdU incorporation in cells transfected with scrambled siRNA, C/EBP siRNA ( H ), KIFC1 siRNA ( I ), or treated with CW069 ( J ). Absorbance was measured at 450–540 nm. A : HCC70, B : MFM223, C : HCC1806, D : BT20; A , B -AR-positive TNBC, C , D-AR-negative TNBC. Bars indicate mean ± SEM. Unpaired two-tailed Student’s t -test with Welch’s correction was used to determine statistical significance. * P < 0.05, ** P < 0.005, ns = non-significant

    Techniques Used: Immunofluorescence, Transfection, BrdU Incorporation Assay, Two Tailed Test



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    Silencing of C/EBPβ and KIFC1 reduces <t>proliferation</t> in AR-TNBC cells. ( A – G ) Representative immunofluorescence images ( A – D ) and quantification bar graphs ( E – G ) showing <t>BrdU</t> (green) incorporation in AR + TNBC ( A , B ) and AR-TNBC ( C , D ) cells transfected with scrambled or C/EBPβ siRNA ( A – D , E ) or treated with CW069 ( A – D , G ). Nuclei were counterstained with Hoechst (blue) and α-tubulin (red). ( H – J ) Bar graphs showing BrdU incorporation in cells transfected with scrambled siRNA, C/EBP siRNA ( H ), KIFC1 siRNA ( I ), or treated with CW069 ( J ). Absorbance was measured at 450–540 nm. A : HCC70, B : MFM223, C : HCC1806, D : BT20; A , B -AR-positive TNBC, C , D-AR-negative TNBC. Bars indicate mean ± SEM. Unpaired two-tailed Student’s t -test with Welch’s correction was used to determine statistical significance. * P < 0.05, ** P < 0.005, ns = non-significant
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    Silencing of C/EBPβ and KIFC1 reduces <t>proliferation</t> in AR-TNBC cells. ( A – G ) Representative immunofluorescence images ( A – D ) and quantification bar graphs ( E – G ) showing <t>BrdU</t> (green) incorporation in AR + TNBC ( A , B ) and AR-TNBC ( C , D ) cells transfected with scrambled or C/EBPβ siRNA ( A – D , E ) or treated with CW069 ( A – D , G ). Nuclei were counterstained with Hoechst (blue) and α-tubulin (red). ( H – J ) Bar graphs showing BrdU incorporation in cells transfected with scrambled siRNA, C/EBP siRNA ( H ), KIFC1 siRNA ( I ), or treated with CW069 ( J ). Absorbance was measured at 450–540 nm. A : HCC70, B : MFM223, C : HCC1806, D : BT20; A , B -AR-positive TNBC, C , D-AR-negative TNBC. Bars indicate mean ± SEM. Unpaired two-tailed Student’s t -test with Welch’s correction was used to determine statistical significance. * P < 0.05, ** P < 0.005, ns = non-significant
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    Representative immunofluorescence images ( A ) and bar graphs ( B ) showing <t>BrdU</t> staining in control and KIFC1 KO AA and EA TNBC cell lines. ( C , D ) Representative images ( C ) and bar graphs ( D ) showing invading control and KIFC1 KO AA and EA TNBC cells stained with crystal violet. Representative images ( E ) and bar graphs ( F ) showing wound closure in control and KIFC1 KO AA and EA TNBC cells. ns-non-significant, **- p < 0.005
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    Image Search Results


    Silencing of C/EBPβ and KIFC1 reduces proliferation in AR-TNBC cells. ( A – G ) Representative immunofluorescence images ( A – D ) and quantification bar graphs ( E – G ) showing BrdU (green) incorporation in AR + TNBC ( A , B ) and AR-TNBC ( C , D ) cells transfected with scrambled or C/EBPβ siRNA ( A – D , E ) or treated with CW069 ( A – D , G ). Nuclei were counterstained with Hoechst (blue) and α-tubulin (red). ( H – J ) Bar graphs showing BrdU incorporation in cells transfected with scrambled siRNA, C/EBP siRNA ( H ), KIFC1 siRNA ( I ), or treated with CW069 ( J ). Absorbance was measured at 450–540 nm. A : HCC70, B : MFM223, C : HCC1806, D : BT20; A , B -AR-positive TNBC, C , D-AR-negative TNBC. Bars indicate mean ± SEM. Unpaired two-tailed Student’s t -test with Welch’s correction was used to determine statistical significance. * P < 0.05, ** P < 0.005, ns = non-significant

    Journal: Cell Communication and Signaling : CCS

    Article Title: C/EBPβ increases tumor aggressiveness by enhancing KIFC1 expression in androgen receptor negative triple negative breast cancer

    doi: 10.1186/s12964-025-02243-7

    Figure Lengend Snippet: Silencing of C/EBPβ and KIFC1 reduces proliferation in AR-TNBC cells. ( A – G ) Representative immunofluorescence images ( A – D ) and quantification bar graphs ( E – G ) showing BrdU (green) incorporation in AR + TNBC ( A , B ) and AR-TNBC ( C , D ) cells transfected with scrambled or C/EBPβ siRNA ( A – D , E ) or treated with CW069 ( A – D , G ). Nuclei were counterstained with Hoechst (blue) and α-tubulin (red). ( H – J ) Bar graphs showing BrdU incorporation in cells transfected with scrambled siRNA, C/EBP siRNA ( H ), KIFC1 siRNA ( I ), or treated with CW069 ( J ). Absorbance was measured at 450–540 nm. A : HCC70, B : MFM223, C : HCC1806, D : BT20; A , B -AR-positive TNBC, C , D-AR-negative TNBC. Bars indicate mean ± SEM. Unpaired two-tailed Student’s t -test with Welch’s correction was used to determine statistical significance. * P < 0.05, ** P < 0.005, ns = non-significant

    Article Snippet: BrdU cell proliferation kit , 2750 , EMD Millipore.

    Techniques: Immunofluorescence, Transfection, BrdU Incorporation Assay, Two Tailed Test

    Representative immunofluorescence images ( A ) and bar graphs ( B ) showing BrdU staining in control and KIFC1 KO AA and EA TNBC cell lines. ( C , D ) Representative images ( C ) and bar graphs ( D ) showing invading control and KIFC1 KO AA and EA TNBC cells stained with crystal violet. Representative images ( E ) and bar graphs ( F ) showing wound closure in control and KIFC1 KO AA and EA TNBC cells. ns-non-significant, **- p < 0.005

    Journal: Cell Communication and Signaling : CCS

    Article Title: A novel role for KIFC1-MYH9 interaction in triple-negative breast cancer aggressiveness and racial disparity

    doi: 10.1186/s12964-024-01664-0

    Figure Lengend Snippet: Representative immunofluorescence images ( A ) and bar graphs ( B ) showing BrdU staining in control and KIFC1 KO AA and EA TNBC cell lines. ( C , D ) Representative images ( C ) and bar graphs ( D ) showing invading control and KIFC1 KO AA and EA TNBC cells stained with crystal violet. Representative images ( E ) and bar graphs ( F ) showing wound closure in control and KIFC1 KO AA and EA TNBC cells. ns-non-significant, **- p < 0.005

    Article Snippet: A BrdU cell proliferation kit (EMD Millipore, #2750) was used to assess cell proliferation.

    Techniques: Immunofluorescence, BrdU Staining, Control, Staining